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湯文強
發(fā)布時間：2017-04-18
一、工作和學習經(jīng)歷
1990.9-1994.6 蘭州大學生物系細胞生物學專業(yè) 學士
1994.7-1998.1 河北師范大學生物系 實習研究員
1999.1-2004.5 美國德克薩斯大學奧斯汀分校植物系 博士
2004.8-2009.9 美國斯坦福大學卡耐基研究院植物系 博士后
2014.8-2015.8 美國加州大學舊金山分校藥學系質譜中心 訪問學者
2010.1至今 河北師范大學生命科學學院 教授
二、研究方向:
目前實驗室的研究興趣主要集中在以下兩個方面：
(1) 油菜素內酯對植物生長發(fā)育調控的作用機理。
作為植物的主要生長調節(jié)激素之一，BR 在植物生長發(fā)育的各個時期都發(fā)揮著重要作用。生理學實驗表明，外加BR 能誘導廣普的植物生理反應，包括種子萌發(fā)，胚軸和莖的生長，木質部的分化，葉片卷曲和偏上生長(epinasty)，根的生長和向重力反應，植物抗病和逆境脅迫反應等等。近年來的研究也發(fā)現(xiàn)通過分子生物學手段在水稻等作物不同的組織中有選擇地增強或減弱內源BR的信號可以成為作物品種改良的一種新的手段和方向。
利用雙子葉模式植物擬南芥為材料，對于 BR 信號轉導途徑的研究取得了許多突破性的進展，并得出一個基本清晰的路徑圖。在該路徑中，BR和質膜受體BRI1結合后促進BRI1和共受體BAK1相互磷酸化，并通過質膜上蛋白激酶BSK或CDG1調節(jié)下游蛋白磷酸酶BSU1的活性。BSU1將蛋白激酶BIN2去磷酸化從而抑制BIN2對轉錄因子BZR1/BES1的磷酸化作用。磷酸化的BZR1/BES1被蛋白磷酸酶PP2A去磷酸化，結合在DNA上，調節(jié)BR相關的基因表達。雖然目前關于BR信號傳遞途徑的研究取得了巨大的進展，對于其中的一些關鍵步驟的調節(jié)機制我們了解得還是不太清楚。因此我們擬通過對這些BR上游信號傳遞蛋白的相互作用組（interactome）的研究來尋找它們的相互作用蛋白，同時篩選這些組分家族的T-DNA插入突變體來進一步揭示BR信號傳遞途徑的調節(jié)機制，為BR信號調節(jié)植物生長發(fā)育的分子機理打下基礎。
（2）植物熱激信號傳導機理。
隨著人類工業(yè)化進程的加劇，溫室效應導致的全球溫度升高是一個不爭的事實。最近30多年來，全球平均溫度已經(jīng)升高了近1度。統(tǒng)計學研究發(fā)現(xiàn)全球平均溫度每升高1度，將導致水稻減產(chǎn)3.2%，玉米減產(chǎn)7.4%，大豆減產(chǎn)3.1%，小麥減產(chǎn)6%。因此研究植物抵抗高溫的分子機制將有利于開發(fā)重要植物耐熱標記基因，幫助培育高產(chǎn)耐熱作物新品種，具有重要的理論和生產(chǎn)應用價值。我實驗室主要關注植物如何感受環(huán)境高溫的變化，并調節(jié)基因表達來提高植物耐熱性的早期熱激信號傳導機制。實驗室前期工作已建立早期熱激信號被激活的分子評估體系，目前正在尋找熱激信號傳導新組分，尤其是能調節(jié)HSFA1s蛋白活性和蛋白穩(wěn)定性的新組分，并解析植物早期熱激記憶形成的分子機制。
三、承擔科研項目：
1. 國家級項目
1) 國家自然科學基金面上項目（2011-2013, 2017-2020, 2019-2022, 2021-2024,2023-2026, 2025-2028，主持）
2) “植物激素作用的分子機理”重大研究計劃集成項目（2015，主持）
3) “植物激素作用的分子機理”重大研究計劃培育項目（2010-2012，主持）
4) “植物胚胎和種子發(fā)育的機理研究”973子課題（2014-2018，參加）
5) 農(nóng)業(yè)部轉基因重大專項子課題（2010, 2013-2015，參加）
2. 省級項目
1) 河北省自然科學基金重點項目（2020-2022，主持）
2) 河北省應用基礎研究計劃重點基礎研究項目（2015-2017，主持）
3) 河北省高等學校創(chuàng)新團隊領軍人才培育計劃（2014-2016，主持）
4) 河北省百人計劃（2012-2016，主持）
5) 河北省杰出青年項目（2011-2013，主持）
6) 河北省百名優(yōu)秀創(chuàng)新人才支持計劃（2010-2012，主持）
7) 河北省留學回國基金（2010-2012，主持）
四、承擔教學任務：
1. 本科生《細胞生物學》雙語課
2. 研究生《細胞信號傳導》
五、所獲專利:
1. “擬南芥HIRK1基因及其同源基因在提高植物耐熱能力方面的應用”，專利號(ZL202210486577.2), 申請日：2022/05/06；授權公告日：2024/6/7；發(fā)明人：任卉敏、湯文強
2. “調節(jié)谷子種子大小的基因及其編碼的蛋白質和應用”，專利號(ZL202011101245.5), 申請日：2020/10/15；授權公告日：2022/7/19；發(fā)明人：湯文強、趙志英
3. “GBP蛋白及其編碼基因在調控植物產(chǎn)量中的應用”, 專利號(ZL201710532910.8)，申請日：2017/7/3；授權日：2020/12/29；發(fā)明人：湯文強、安志超、張寶文
4. “水稻敏熱基因UBP21的應用”，專利號(ZL201410745025.4), 申請日：2014/12/09； 授權日：2018/9/18；發(fā)明人：湯文強、張寶文、安志超
5. "水稻基因BSK3的應用”，專利號(ZL201410832924.8), 申請日：2014/12/29；授權日：2017/11/14；發(fā)明人：湯文強、張寶文
6. “Newisolated nucleic acid molecule comprising a sequence of nucleotides thatencodes a brassinosteroid receptor regulated kinase useful for producingtransgenic plants and modulating cellular process of a plant cell”. 專利號：WO2010011285-A2;US2010043097-A1. 發(fā)明人：Wenqiang Tang and Zhiyong Wang
六、所獲獎勵：
1. “油菜素內酯信號傳導及其調控植物生長發(fā)育的分子機制”項目獲2020年度河北省科學技術獎(自然科學獎)一等獎
七、代表性論文
1. ZhangY, Han L, Liu J, Chang M, Li C, Shang J, Deng Z*, Tang W*,Sun Y* (2025) Two E-clade Protein Phosphatase 2Cs enhance ABAsignaling by dephosphorylating ABI1 in Arabidopsis. Molecular Plant(accepted)
該論文發(fā)現(xiàn)了兩個能將ABI1第117位絲氨酸去磷酸化來調節(jié)ABI1蛋白穩(wěn)定性和活性的E家族PP2C蛋白磷酸酶，研究結果為解析ABA信號傳導的調控機制提供了新的調控組分。
2. Li B,Jiang S, Gao L, Wang W, Luo H, Dong Y, Gao Z, Zheng S, Liu X*, Tang W*(2024) Heat Shock Factor A1s are required for phytochrome-interaciing factor4-mediated thermomorphogenesis in Arabidopsis. J Integr Plant Biol.66(1):20-35 (Cover story)
該論文發(fā)現(xiàn)HSFA1能通過調節(jié)PIF4的轉錄和蛋白穩(wěn)定性調節(jié)植物的高溫形態(tài)建成過程，證明了植物溫和高溫和熱激高溫響應不是兩個獨立的生物學過程。
3. Wu X,Cai X, Zhang B, Wu S, Wang R, Li N, Li Y, Sun Y*, Tang W* (2022) ERECTAregulates seed size independently of its intracellular domain viaMAPK-DA1-UBP15 signaling. Plant Cell 34(10): 3773-3789
該論文發(fā)現(xiàn)了種子發(fā)育過程中母體遺傳調控珠被細胞分裂的近完整的信號通路：ER-MAPK-DA1-UBP15。
4. Wang R, Wang R, Liu M, Yuan W, Zhao Z, Liu X,Peng Y, Yang X, Sun Y, Tang W*(2021) Nucleocytoplasmic trafficking and turnover mechanisms of BRASSINAZOLE RESISTANT 1 in Arabidopsis thaliana. Proc. Natl. Acad.Sci. U.S.A. 118(33):e2101838118
該論文證明了BR能迅速將胞質中磷酸化的BZR1招募到細胞核中，并在細胞核中去磷酸化，同時還提出了去磷酸化的BZR1也能被26S蛋白酶體途徑降解。
5. Li B,Gao Z, Liu X, Sun D, Tang W* (2019)Transcriptional Profiling Reveals a Time-of-Day-Specific Role of REVEILLE 4/8in Regulating the First Wave of Heat Shock–Induced Gene Expression inArabidopsis. Plant Cell 31: 2353-2369
該論文發(fā)現(xiàn)生物鐘核心調控元件RVE4/8能獨立于HSFA1s途徑快速調節(jié)熱激高溫響應基因的表達，調節(jié)植物在中午時對環(huán)境高溫的耐受能力。
6. Chen L,Gao Z, Zhao Z, Liu X, Li Y, Zhang Y, Liu X, Sun Y and Tang W* (2019) BZR1 Family Transcription Factors FunctionRedundantly and Indispensably in BR Signaling but Exhibit BRI1-IndependentFunction in Regulating Anther Development in Arabidopsis. Molecular Plant 12:1408-1415
該論文證明了BZR1家族轉錄因子是BR信號途徑不可缺失的組分
7. Li B,Gao K, Ren H and Tang W*. (2018)Molecular mechanisms governing plant responses to high temperatures. JIntegr Plant Biol. 60(9):757-779 (Invited Expert Review)
該論文系統(tǒng)論述了植物響應高溫的信號途徑，多次入選ESI高被引論文。
8. An Z, Liu Y, Ou Y, Li J, Zhang B, Sun D,Sun Y*, Tang W*. (2018) Regulation of the stability of RGF1 receptor bythe ubiquitin-specific proteases UBP12/UBP13 is critical for root meristemmaintenance. Proc. Natl. Acad. Sci. U.S.A. 115(5):1123-1128
該論文發(fā)現(xiàn)了一個RGF1-RGIs信號途徑新成員UBP12/13，證明UBP12/13能通過將RGI1去泛素化，調節(jié)植物根對RGF1的響應。
9. Wang R,Liu M, Yuan M, Oses-Prieto JA, Cai X, Sun Y, Burlingame A, Wang Z, Tang W*.(2016) The brassinosteroid activated BRI1 receptor kinase is switched off bydephosphorylation mediated by cytoplasm-localized PP2A B’ subunits. MolecularPlant 9(1):148-157.
該論文發(fā)現(xiàn)PP2A能將BRI1去磷酸化關閉BR信號，并提出了BZR1可能在細胞核中被去磷酸化的觀點。
10. Tang W*, YuanM*, Wang R*, Yang Y*, Wang C, Oses-Prieto JA, Kim TW, Zhou H, Deng Z, GampalaSS, Gendron JM, Jonassen EM, Lillo C, DeLong A, Burlingame AL, Sun Y &Wang ZY. (2011) PP2A activates brassinosteroid responsivegene expression and plant growth by dephosphorylating BZR1. NatureCell Biology 13(2):124-131. (*equal contribution)
該論文發(fā)現(xiàn)了一個BR信號傳導途徑的新成員PP2A，證明PP2A能將BZR1去磷酸化激活BR信號途徑。
11. Tang W, KimTW, Oses-Prieto JA, Sun Y, Deng Z, Zhu S,Wang R, Burlingame AL, and Wang ZY (2008) BSKs mediate signaltransduction from the receptor kinase BRI1 in Arabidopsis. Science 321(5888):557-560.
該論文發(fā)現(xiàn)了一個BR信號傳導途徑的新成員BSK1/2, 證明BSK1/2作用在受體BRI1的下游將BR信號從細胞膜上傳遞到細胞質中。
八、其他發(fā)表論文
12. Ning K,Li X, Yan J, Liu J, Gao Z, Tang W*, Sun Y* (2025) Heat stress inhibitspollen development by degrading mRNA capping enzyme ARCP1 and ARCP2. PlantCell Environ. 48(2):978-991
13. Cai X,Lee S, Jaime APG, Tang W, Sun Y, Huq E* (2024) Phosphatase 2Adephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesisin Arabidopsis. Plant Cell. 36(10)4457-4471.
14. Shi L,Li C, Lv G, Li X, Feng W, Bi Y, Wang W, Wang Y, Zhu L, Tang W, Fu Y*(2024) The adaptor protein ECAP, the corepressor LEUNIG, and the transcriptionfactor BEH3 interact and regulate microsporocyte generation in Arabidopsis. PlantCell. 36(7) 2531-2549
15. Li S,Yan J, Chen LG, Meng G, Zhou Y, Wang CM, Jiang L, Luo J, Jiang Y, Li QF, TangW*, He JX* (2024) Brassinosteroid regulates stomatal development inetiolated cotyledons via transcription factors BZR1 and BES1. PlantPhysiol. 195(2):1382-1400
16. Li C,Li X, Deng Z, Song Y, Liu X, Tang X, Li Z, Zhang Y, Zhang B, Tang W,Shang J* and Sun Y* (2024) EGR1 and EGR2 positively regulate plant ABAsignaling by modulating the phosphorylation of SnRK2.2. New Phytol.241(4): 1492-1509
17. Tang S,Zhao Z, Liu X, Sui Y, Zhang D, Zhi H, Gao Y, Zhang H, Zhang L, Wang Y, Zhao M,Li D, Wang K, He Q, Zhang R, Zhang W, Jia G, Tang W, Ye X, Wu C*, Diao X*(2023) An E2-E3 pair contributes to seed size control in grain crops. NatCommun. 14(1):3091
18. Wang D,Li Y, Wang H, Xu Y, Yang Y, Zhou Y, Chen Z, Zhou Y, Gui L, Guo Y, Zhou C, TangW, Zheng S, Wang L, Guo X, Zhang Y, Cui F, Lin X, Jiao Y, He Y, Li J, He F,Liu X*, Xiao J* (2023) Boosting wheat functional genomics via an indexed EMSmutant libraray of KN9204. Plant Commun. 4(4)100593
19. Ren H,Wu X, Zhao W, Wang Y, Sun D, Gao K*,Tang W* (2022) Heat Shock-InducedAccumulation of the Glycogen Synthase Kinase 3-Like Kinase BRASSINOSTEROIDINSENSITIVE 2 Promotes Early Flowering but Reduces Thermotolerance inArabidopsis. Front Plant Sci. 13:838062
20. Zhang H,Guo L, Li Y, Zhao D, Liu L, Chang W, Zhang K, Zheng Y, Hou J, Fu C, Zhang Y,Zhang B, Ma Y, Niu Y, Zhang K, Sing J, Cui S, Wang F, Tan K, Zheng S, Tang W, Dong J*, Liu X* (2022)TOP1a fine-tunes TOR-PLT2 to maintain root tip homeostasis in response tosugars. Nature Plant 8(7):792-801
21. Li M,Li P, Wang C, Xu H, Wang M, Wang Y, Niu X, Xu M, Wang H, Qin Y, Tang W, Bai M, Wang W, Wu S (2022)Brassinosteroid signaling restricts root lignification by antagonizingSHORT-ROOT function in Arabidopsis. Plant Physiology 190(2):1182-1198
22. Zhao Z, Tang S, Li W, Yang X, Wang R, Diao X*, Tang W* (2021) Overexpressionof a BRASSINAZOLE RESISTANT 1 homolog attenuates drought tolerance bysuppressing the expression of PLETHORA-LIKE 1 in Setaria italica.Crop J. 9(5):1208-1213
23. Tian Y,Zhao N, Wang M, Zhou W, Guo J, Han C, Zhou C, Wang W, Wu S, Tang W, FanM, Bai M (2021) Integrated regulation of periclinal cell division bytranscriptional module of BZR1-SHR in Arabidopsis roots. New Phytologist 233(2):795-808
24. Zho Z, Tang S, Zhang Y, Yue J, Xu J, Tang W, Sun Y,Wang R, Diao X*, Zhang B* (2021) Evolutionary analysis and functionalcharacterization of SiBRI1 as a brassinosteroid receptor gene in foxtailmillet. BMC Plant Biology 21:291
25. Tian X,He M, Mei E, Zhang B, Tang J, Xu M, Liu J, Li X, Wang Z, Tang W, Bu Q* (2021) WRKY53Integrates Classic Brassinosteroid Signaling and the Mitogen-Activated ProteinKinase Pathway to Regulate Rice Architecture and Seed Size. PlantCell 33(8): 2753-2775
26. Zhao M,Tang S, Zhang H, He M, Liu J, Zhi H, Sui Y, Liu X, Jia G, Zhao Z, Yan J, ZhangB, Zhou Y, Chu J, Wang X, Zhao B, Tang W, Li J, Wu C*, Liu X* andDiao X* (2020)DROOPY LEAF1 controls leaf architecture by orchestrating early brassinosteroidsignaling. Proc. Natl. Acad. Sci. U.S.A. 117(35):21766-21774
27. Jia D,Chen LG, Yin G, Yang X, Gao Z, Guo Y, Sun Y and Tang W* (2020)Brassinosteroids regulate outer ovule integument growth in part via the controlof INNER NO OUTER by BRASSINOZOLE-RESISTANT family transcription factors. JIntegr Plant Biol. 62(8):1093-1111 (Cover story)
28. Zhou H,Wang X, Huo C, Wang H, An Z, Sun D, Liu J, TangW* and Zhang B* (2019) AQuantitative Proteomics Study of Early Heat-Regulated Proteins byTwo-Dimensional Difference Gel Electrophoresis Identified OsUBP21 as a NegativeRegulator of Heat Stress Responses in Rice. Proteomics 19:e1900153 (*co-corresponding authors, Cover story)
29. Gao Z,Zhao Z, Tang W* (2018) DREAMSeq: animproved method for analyzing differentially expressed genes in RNA-seq data. Frontiers in Genetics 9:588
30. 霍晨敏、湯文強 (2016) 植物冷信號傳導機制研究進展 生物技術通報 10:27-33 (特約綜述)
31. Yang X,Bai Y, Shang J, Xin R, Tang W*.(2016) The Antagonistic Regulation of Abscisic Acid-Inhibited Root Growth byBrassinosteroids is Partially Mediated via Direct Suppression of ABSCISIC ACIDINSENSITIVE 5 Expression by BRASSINAZOLE RESISTANT 1. Plant, Cell & Environment39(9):1994-2003
32. Zhang B,Wang X, Zhao Z, Wang R, Huang X, Zhu Y, Yuan L, Wang Y, Xu X, Burlingame AL, GuoY, Sun Y, Tang W*. (2016) OsBRI1 activates BR signaling by preventingbinding between the TPR and kinase domains of OsBSK3 via phosphorylation. PlantPhysiology 170(2):1149-1161.
33. Huo C, ZhangB, Wang H, Wang F, Liu M, Gao Y, Zhang W, Deng Z, Sun D, Tang W*. (2016)Comparative study of early cold-regulated proteins by two dimentionaldifference gel electrophoresis reveals a key role for phospholipase Da1 inmediating cold acclimation signaling pathway in rice. Molecular &Cellular Proteomics 15(4):1397-1411.
34. Wang X， Ma X, Wang H, LiB, Clark G, Guo Y, Roux S, Sun D and Tang W*, (2015) Proteomic study ofmicrosomal proteins reveals a key role for Arabidopsis Annexin 1 in mediatingheat stress-induced increase in intracellular calcium levels. Molecular& Cellular Proteomics 14(3):686-694.
35. Xu P,Xu S, Li Z, Tang W, BurlingameAL and Wang ZY (2014) A brassinosteroid signaling kinase interacts withmultiple receptor-like kinases in Arabidopsis. Molecular Plant 7(2):441-444.
36. Tang W* (2012) Quantitative proteomic analysis ofplasma membrane using two-dimensional difference gel electrophoresis (2-DDIGE). Methods in Molecular Biology 876:67-82.
37. Liu X,Wu J, Clark G, Lundy S, Lim M, Arnold D, Chan J, Tang W, Muday GK,Gardner G, Roux SJ (2012). Role for apyrases in polar auxin transport inArabidopsis. Plant Physiology 160(4):1985-1995
38. SamuelMA, Tang W, Jamshed M, Northey J, Patel D, Smith D, Siu M, MuenchDG, Wang ZY, and Goring DR (2011) Proteomic analysis of Brassica stigmaticproteins following the self-incompatibility reaction reveals a role formicrotubule dynamics during pollen responses. Molecular & CellularProteomics 10(12):M111.011338.
39. Tang W, Deng Z and Wang ZY (2010)Proteomics shed light on the brassinosteroid signaling mechanism. CurrentOpinion in Plant Biology 13(1):27-33.
40. Sun Y,Fan X, Cao D, Tang W, He K, Zhu J, He JX, Bai MY, Zhu S, Oh E, Patil S,Kim TW, Ji H, Wong W, Rhee S, Wang ZY (2010) Integration of BrassinosteroidSignal Transduction with the Transcription Network for Plant Growth Regulationin Arabidopsis. Development Cell 19(5):765-777.
41. Kim TW,Guan S, Sun Y, Deng Z, Tang W,Shang J, Sun Y, Burlingame AL and Wang ZY (2009) Brassinosteroid signaltransduction from cell-surface receptor kinases to nuclear transcriptionfactors. Nature Cell Biology 11(10):1254-1262.
42. Tang W, Deng Z,Oses-Prieto JA, Suzuki N, Zhu S, Zhang X, Burlingame AL, and Wang ZY (2008) Proteomic studies of brassinosteroid signaltransduction using prefractionation and 2-D DIGE. Molecular &Cellular Proteomics 7(4):728- 738.
43. Deng Z,Zhang X, Tang W, Oses-Prieto JA, Suzuki N, Gendron JM, Chen H, Guan S, ChalkleyRJ, Peterman TK, Burlingame AL and Wang ZY (2007) A proteomic study ofbrassinosteroid response in Arabidopsis. Molecular & CellularProteomics 6(12):2058-2071
44. GampalaSS, Kim TW, He J, Tang W,Deng Z, Bai M, Guan S, Lalonde S, Sun Y, Gendron JM, Chen H, Shibagaki N, FerlRJ, Ehrhardt D, Chong K, Burlingame AL and Wang ZY (2007) An Essential Role for14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis. DevelopmentalCell 13(2):177-189
45. Tang W, Brady SR, Sun Y,Muday GK, and Roux SJ (2003) ExtracellularATP Inhibits Root Gravitropism at Concentrations That Inhibit Polar AuxinTransport. PlantPhysiology 131, 147-154
46. Jeter C*,Tang W*, Henaff E, Butterfield T, and Roux SJ (2004) Evidence of a NovelCell Signaling Pathway in Arabidopsis Induced by Extracellular Nucleoside Tri-and Diphosphates. Plant Cell 16, 2652- 2664 (*equal contribution)
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